사람 SH-SY5Y 신경아세포종 에서 CoCl2에 의한 세포고사에 LED 조사의 영향
Effects of LED irradiation on the expression of apoptosis-related molecules in human SH-SY5Y neuroblastoma cells
조규승, 유선열, 최홍란,
소속 상세정보
조규승 ( Cho Kyu-Seung ) - 전남대학교 치과대학 구강악안면외과학교실
유선열 ( Ryu Sun-Youl ) - 전남대학교 치의학전문대학원 구강악안면외과학교실
최홍란 ( Choi Hong-Ran ) - 전남대학교 치과대학 구강병리학교실
KMID : 0355620070330010001
Abstract
To verify the inhibitory or protective effects of light-emitting diode(LED) irradiation on apoptotic cell death induced by CoCl2, human SH-SY5Y cells were treated with CoCl2 and LED were used to irradiate the cells. In the cell viability assay, cells were died slowly from 50 ?M to 250 ?M and about 50% of cells died after 12 hours at 400 ?M of CoCl2. The Diff-Quik staining revealed that cells showed condensation of DNA and blebbing of the cell membrane. The DNA fragmentation assay revealed the DNA fragmentation, which is another apoptosis marker, occurred in cells treated with 400 ?M CoCl2 for 16 hours. In the western blot for HIF-1?, HIF-1?was expressed after 3 hours from induction and peaked maximally at 16 hours. In the cell viability assay of the effects of LED irradiation (at 590 nm for 1 hour 20 minutes), the cells showed more proliferation (about 20%) than the control group. The RPA assay of various apoptosis-related molecules showed that pro-apoptosis molecules such as Bax, Bak, and Bid were upregulated in the CoCl2 treatment group. This means that the apoptotic cell population was increased. However there was some significant changes in LED irradiated cells. In the CoCl2- treated LED irradiation group, those molecules were down-regulated more than in the only CoCl2-treated group. These results have shown that CoCl2 may induce apoptotic cell death in human SH-SY5Y neuroblastoma cells. And LED irradiation has a positive effect on apoptotic cells by down-regulation of pro-apoptotic molecules.
키워드
LED irradiation;Apoptotic cell death;Human SH-SY5Y cells
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